Crystallization and preliminary X-ray diffraction studies of oxidized flavodoxin from Chondrus crispus, a red alga.
J Biochem
; 105(3): 348-50, 1989 Mar.
Article
em En
| MEDLINE
| ID: mdl-2732209
ABSTRACT
Crystals of the oxidized form of flavodoxin from a red alga, Chondrus crispus, have been grown in ammonium sulfate solution by the dialysis method. The crystals belong to the orthorhombic system, space group P2(1)2(1)2(1), with unit cell dimensions of a = 63.6, b = 48.8, and c = 56.8 A. The asymmetric unit contains one molecule of flavodoxin. The crystals diffract X-rays to about 2.0 A resolution and are stable to X-ray beams. The diffraction patterns changed significantly upon soaking the crystal in a solution of a platinum complex. The major heavy-atom sites in the platinum derivative crystal have been identified from the difference Patterson function calculated at 4 A resolution.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Rodófitas
/
Flavodoxina
/
Flavoproteínas
Idioma:
En
Revista:
J Biochem
Ano de publicação:
1989
Tipo de documento:
Article