MR targeted imaging for the expression of tenascin-C in myocardial infarction in vivo.
J Magn Reson Imaging
; 45(6): 1668-1674, 2017 06.
Article
em En
| MEDLINE
| ID: mdl-27865025
ABSTRACT
PURPOSE:
To investigate the presence of viable myocardium in mice with acute myocardial infarction (MI) using a molecular targeted probe. MATERIALS ANDMETHODS:
Super paramagnetic iron oxide (SPIO) nanoparticles and tenascin-C antibody were conjugated as an MRI probe. Fifteen mice with infarction were injected with SPIO-anti-tenascin-C (3 days [d], 5d, 7d after infarction; n = 5 for each group). Another five mice with infarction (5d, n = 5) were injected with SPIO for comparison. In vivo MR (7 Tesla, fast low-angle shot multi-slice T2* sequence) was performed for tracing. Histological analysis was used to compare surviving cardiomyocytes with signal changes on MR.RESULTS:
The mRNA expression of tenascin-C increased directly after MI and peaked at the fifth day (5d 24.29 ± 1.41 versus 3d 10.63 ± 0.72, 7d 6.56 ± 0.12; P < 0.01). T2 relaxation rate of synthesized SPIO-anti-tenascin-C was r2 = 338 mM-1 s-1 . After MR, the signal changes (contrast-to-noise ratio) of the research group were 3d 6.51 ± 1.13 versus 5d 14.06 ± 3.19 versus 7d 5.02 ± 2.65, P < 0.05. The MR signal showed a small decrease in the contrast group on 5d (research group 14.06 ± 3.19 versus contrast group 1.75 ± 0.59, P < 0.05).CONCLUSION:
Tenascin-C was expressed by surviving cardiomyocytes within the infarcted region. MR imaging with SPIO-anti-tenascin-C might be used to evaluate myocardial viability of MI patients before therapy. LEVEL OF EVIDENCE 1 Technical Efficacy Stage 4 J. MAGN. RESON. IMAGING 2017;451668-1674.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imageamento por Ressonância Magnética
/
Miocárdio Atordoado
/
Tenascina
/
Imagem Molecular
/
Infarto do Miocárdio
Tipo de estudo:
Diagnostic_studies
/
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
J Magn Reson Imaging
Assunto da revista:
DIAGNOSTICO POR IMAGEM
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
China