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Generation of a TLE1 homozygous knockout human embryonic stem cell line using CRISPR-Cas9.
Herring, Amanda; Messana, Angelica; Bara, Anne M; Hazelbaker, Dane Z; Eggan, Kevin; Barrett, Lindy E.
Afiliação
  • Herring A; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Messana A; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Bara AM; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Hazelbaker DZ; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Eggan K; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA.
  • Barrett LE; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA. Electronic address: lbarrett@broadinstitute.org.
Stem Cell Res ; 17(2): 430-432, 2016 09.
Article em En | MEDLINE | ID: mdl-27879218
ABSTRACT
Here, we generated a biallelic mutation in the TLE1 (Transducin Like Enhancer of Split 1) gene using CRISPR-Cas9 editing in the human embryonic stem cell (hESC) line WA01. The homozygous knockout cell line, TLE1-464-G04, displays loss of TLE1 protein expression while maintaining pluripotency, differentiation potential and genomic integrity.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Repressoras / Sistemas CRISPR-Cas / Células-Tronco Embrionárias Humanas Limite: Humans / Male Idioma: En Revista: Stem Cell Res Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Repressoras / Sistemas CRISPR-Cas / Células-Tronco Embrionárias Humanas Limite: Humans / Male Idioma: En Revista: Stem Cell Res Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Estados Unidos