Production of lipid-containing microalgal biomass and simultaneous removal of nitrate and phosphate from synthetic wastewater.

The major concerns of the modern society such as increasing population, climate change and economic development are imposing continuous stress on water and energy resources. The present work deals with the cultivation of green algae Desmodesmus abundans for optimum biomass productivity and lipid content as well as simultaneous removal of nitrate and phosphate from synthetic wastewater. The algal biomass is characterized by ultimate analysis, scanning electron microscopic analysis and thermogravimetric analysis. The effect of time, inoculum concentration and nitrate concentration on four responses (biomass productivity, lipid content, removal of nitrate and removal of phosphate) are studied by response surface methodology using central composite design. The quadratic models are found to be suitable for each response. At optimized experimental conditions, the algae showed biomass productivity of 46.96 mg L-1 day-1, lipid content of 16.23%, nitrate removal of 86.64% and phosphate removal of 87.52% after 27 days, when the initial inoculum concentration was 6% and nitrate concentration was 1.25 g L-1.