A CRISPR toolbox to study virus-host interactions.
Nat Rev Microbiol
; 15(6): 351-364, 2017 06.
Article
em En
| MEDLINE
| ID: mdl-28420884
ABSTRACT
Viruses depend on their hosts to complete their replication cycles; they exploit cellular receptors for entry and hijack cellular functions to replicate their genome, assemble progeny virions and spread. Recently, genome-scale CRISPR-Cas screens have been used to identify host factors that are required for virus replication, including the replication of clinically relevant viruses such as Zika virus, West Nile virus, dengue virus and hepatitis C virus. In this Review, we discuss the technical aspects of genome-scale knockout screens using CRISPR-Cas technology, and we compare these screens with alternative genetic screening technologies. The relative ease of use and reproducibility of CRISPR-Cas make it a powerful tool for probing virus-host interactions and for identifying new antiviral targets.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Contexto em Saúde:
3_ND
Problema de saúde:
3_dengue
/
3_neglected_diseases
Assunto principal:
Vírus do Nilo Ocidental
/
Hepacivirus
/
Vírus da Dengue
/
Ensaios de Triagem em Larga Escala
/
Sistemas CRISPR-Cas
/
Zika virus
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Nat Rev Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Estados Unidos