Effect of ZEB2 silencing on cisplatin resistance in gastric cancer.
Eur Rev Med Pharmacol Sci
; 21(8): 1746-1752, 2017 04.
Article
em En
| MEDLINE
| ID: mdl-28485807
ABSTRACT
OBJECTIVE:
To investigate the effect of ZEB2 silencing on cisplatin resistance in gastric cancer. MATERIALS ANDMETHODS:
The resulting cell line, SGC7901/DDP, was transfected with ZEB2 siRNA, non-specific siRNA, or vehicle control. The effectiveness of ZEB2 silencing was evaluated by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot. MTT viability assay was used to determine the cisplatin-sensitivity of cells. Cell apoptosis was measured by flow cytometry.RESULTS:
A significant decrease in ZEB2 in mRNA and protein level was seen in cells transfected with ZEB2 siRNA, compared to that in cells transfected with non-specific siRNA or vehicle. Transfection with ZEB2 siRNA in cisplatin-resistant SGC7901/DDP cells resulted in a significant decrease in cell viability in response to the cisplatin treatment, and cell viability decreased with increasing cisplatin concentrations. A higher apoptotic rate was also seen in cells transfected with ZEB2 siRNA under cisplatin treatment.CONCLUSIONS:
ZEB2 silencing can effectively make gastric cells sensitive to cisplatin treatment in vitro.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Contexto em Saúde:
6_ODS3_enfermedades_notrasmisibles
Problema de saúde:
6_digestive_diseases
/
6_stomach_cancer
Assunto principal:
Neoplasias Gástricas
/
Cisplatino
/
Homeobox 2 de Ligação a E-box com Dedos de Zinco
/
Antineoplásicos
Limite:
Humans
Idioma:
En
Revista:
Eur Rev Med Pharmacol Sci
Assunto da revista:
FARMACOLOGIA
/
TOXICOLOGIA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
China