Development of a rectal sexually transmitted infection (STI) Model in Rhesus macaques using Chlamydia trachomatis serovars E and L2.
J Med Primatol
; 46(5): 218-227, 2017 10.
Article
em En
| MEDLINE
| ID: mdl-28488731
ABSTRACT
BACKGROUND:
Rectal STI coinfection models enhance the understanding of rectal HIV transmission risk factors. MATERIALS ANDMETHODS:
Rhesus macaques (n=9) were exposed to one of three rectal Chlamydia trachomatis (CT) challenges C. trachomatis L2 (CT-L2 ); C. trachomatis serovar E (CT-E), followed by CT-L2 ; or CT-E, treatment/clearance, then CT-L2 . Infections were monitored by PCR. Weekly blood and rectal secretion/lavage samples were collected for cytokine analyzes and/or epithelial sloughing, occult, and overt blood determinations.RESULTS:
Chlamydial infections were successfully established in each animal, with varying degrees of persistence. Mucosal IL-1beta was upregulated in animals consecutively infected with CT-E then CT-L2 (P=.05). Epithelial sloughing was also significantly increased post-infection in this group (P=.0003).CONCLUSIONS:
This study demonstrates successful rectal infection of rhesus macaques with CT-E and CT-L2 and describes measures of assessing rectal inflammation and pathology. Different infection strategies yield varying inflammatory and pathologic outcomes, providing well-described models for future SIV/SHIV susceptibility studies.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções por Chlamydia
/
Infecções Sexualmente Transmissíveis
/
Infecções por HIV
/
Chlamydia trachomatis
/
Modelos Animais de Doenças
/
Macaca mulatta
Tipo de estudo:
Prognostic_studies
/
Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
J Med Primatol
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Estados Unidos