Activity of rosuvastatin in tachyzoites of Toxoplasma gondii (RH strain) in HeLa cells.
Exp Parasitol
; 181: 75-81, 2017 Oct.
Article
em En
| MEDLINE
| ID: mdl-28774497
ABSTRACT
Due to the toxicity of conventional medication in toxoplasmosis, some drugs are being studied for treating this infection, such as statins, especially rosuvastatin compound, which is efficient in inhibiting the initial isoprenoid biosynthesis processes in humans and the parasite. The goal of this study was to assess the activity of rosuvastatin in HeLa cells infected with the RH strain of T. gondii. In the experiment, HeLa cells (1 × 105) were infected with tachyzoites of T. gondii (5 × 105). After the experimental infection, we assessed the number of infected cells and the amount of intracellular tachyzoites. In addition, culture supernatants were collected to determine the amount of cytokines by cytometric bead array. We observed that there was no cytotoxicity in the concentrations tested in this cell line. The effect of rosuvastatin showed a significant reduction in both the number of infected cells and the proliferation index of the intracellular parasite, when compared with the conventional treatment combining sulfadiazine and pyrimethamine for toxoplasmosis. There were also reduced levels of cytokines IL-6 and IL-17. Therefore, it was concluded that rosuvastatin exhibited antiproliferative activity. The data presented are significant to promote further studies and the search for alternative treatment for toxoplasmosis.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Contexto em Saúde:
3_ND
Problema de saúde:
3_neglected_diseases
/
3_zoonosis
Assunto principal:
Toxoplasma
/
Células HeLa
/
Inibidores de Hidroximetilglutaril-CoA Redutases
/
Rosuvastatina Cálcica
Limite:
Humans
Idioma:
En
Revista:
Exp Parasitol
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Brasil