Vitamin C acts as a hepatoprotectant in carbofuran treated rat liver slices in vitro.
Toxicol Rep
; 4: 265-273, 2017.
Article
em En
| MEDLINE
| ID: mdl-28959648
ABSTRACT
Carbamates, most commonly used pesticides in agricultural practices, have been reported to produce free radicals causing deleterious effects in animals. The present study was designed to assess the carbofuran induced oxidative stress in rat liver slices in vitro and also to evaluate protective role of vitamin C by incubating them in Krebs-Ringer HEPES Buffer (KRHB) containing incubation media (Williams medium E (WME) supplemented with glucose and antibiotics) with different concentrations of carbofuran. The results demonstrated that carbofuran caused significant increase in lipid peroxidation and inhibition in the activity of hepatic superoxide dismutase (SOD) in concentration dependent manner. The data with incubation medium reflected that carbofuran at lowest concentration caused an increase in SOD activity followed by its inhibition at higher concentration. Carbofuran treatment caused inhibition in the activity of catalase in liver slices and WME incubation medium. Pre-incubation of liver slices and the WME media with vitamin C restored the values of biochemical indices tested. The results indicated that carbofuran might induce oxidative stress in hepatocytes. The pretreatment with vitamin C may offer hepatoprotection from toxicity of pesticide at low concentration only.
Antioxidant; BSA, Bovine serum albumin; CaCl2, calcium chloride; Carbofuran; CuSO4, copper sulphate; DMSO, Dimethylsulfoxide; EDTA, Ethylenediaminetetraacetic acid; Hepatotoxicity; In vitro; KCl, potassium chloride; KRHB, Krebs-Ringer HEPES Buffer; MgSO4, magnesium sulfate; NADH, nicotinamide adenine dinucleotide; NaCl, sodium chloride; NaOH, sodium hydroxide and MDA Malonaldialdehyde; Oxidative stress; RNS, reactive nitrogen species; ROS, reactive oxygen species; SOD, superoxide dismutase; TBA, thiobarbituric acid; TCA, trichloroacetic acid; WME, Williams medium E
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01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Toxicol Rep
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Índia