The poly(A)-binding protein facilitates in vitro translation of poly(A)-rich mRNA.
Eur J Biochem
; 176(3): 521-6, 1988 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-2901956
ABSTRACT
To investigate the role of the 73-kDa poly(A)-binding protein in protein synthesis, the effect of the addition of homo-polyribonucleotides on the translation of polyadenylated and non-adenylated mRNA was studied in the rabbit reticulocyte lysate. Poly(A) was found to be the most effective polynucleotide in inhibiting duck-globin mRNA translation, whereas it had no effect on the translation of polyribosomal duck-globin mRNP, or on the endogenous synthesis of the rabbit reticulocyte lysate. The translation of poly(A)-free mRNA was not affected by the addition of poly(A). Furthermore, we found that the inhibiting effect of poly(A) can be reversed by addition of purified poly(A)-binding protein. It is thus likely that the 73-kDa poly(A)-binding protein is an essential factor necessary for poly(A)-rich mRNA translation.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poli A
/
Biossíntese de Proteínas
/
RNA Mensageiro
/
Proteínas de Transporte
Limite:
Animals
Idioma:
En
Revista:
Eur J Biochem
Ano de publicação:
1988
Tipo de documento:
Article
País de afiliação:
França