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The p75NTR-mediated effect of nerve growth factor in L6C5 myogenic cells.
de Perini, Alessandra; Dimauro, Ivan; Duranti, Guglielmo; Fantini, Cristina; Mercatelli, Neri; Ceci, Roberta; Di Luigi, Luigi; Sabatini, Stefania; Caporossi, Daniela.
Afiliação
  • de Perini A; Department of Movement, Human and Health Sciences, University of Rome Foro Italico, Piazza Lauro de Bosis, 15, 00135, Rome, Italy.
  • Dimauro I; Department of Movement, Human and Health Sciences, University of Rome Foro Italico, Piazza Lauro de Bosis, 15, 00135, Rome, Italy.
  • Duranti G; Department of Movement, Human and Health Sciences, University of Rome Foro Italico, Piazza Lauro de Bosis, 15, 00135, Rome, Italy.
  • Fantini C; Department of Movement, Human and Health Sciences, University of Rome Foro Italico, Piazza Lauro de Bosis, 15, 00135, Rome, Italy.
  • Mercatelli N; Department of Movement, Human and Health Sciences, University of Rome Foro Italico, Piazza Lauro de Bosis, 15, 00135, Rome, Italy.
  • Ceci R; Laboratory of Cellular and Molecular Neurobiology, CERC, Fondazione Santa Lucia, Via del Fosso di Fiorano, 64, 00143, Rome, Italy.
  • Di Luigi L; Department of Movement, Human and Health Sciences, University of Rome Foro Italico, Piazza Lauro de Bosis, 15, 00135, Rome, Italy.
  • Sabatini S; Department of Movement, Human and Health Sciences, University of Rome Foro Italico, Piazza Lauro de Bosis, 15, 00135, Rome, Italy.
  • Caporossi D; Department of Movement, Human and Health Sciences, University of Rome Foro Italico, Piazza Lauro de Bosis, 15, 00135, Rome, Italy.
BMC Res Notes ; 10(1): 686, 2017 Dec 04.
Article em En | MEDLINE | ID: mdl-29202822
ABSTRACT

OBJECTIVE:

During muscle development or regeneration, myocytes produce nerve growth factor (NGF) as well as its tyrosine-kinase and p75-neurotrophin (p75NTR) receptors. It has been published that the p75NTR receptor could represent a key regulator of NGF-mediated myoprotective effect on satellite cells, but the precise function of NGF/p75 signaling pathway on myogenic cell proliferation, survival and differentiation remains fragmented and controversial. Here, we verified the role of NGF in the growth, survival and differentiation of p75NTR-expressing L6C5 myogenic cells, specifically inquiring for the putative involvement of the nuclear factor κB (NFκB) and the small heat shock proteins (sHSPs) αB-crystallin and Hsp27 in these processes.

RESULTS:

Although NGF was not effective in modulating myogenic cell growth or survival in both standard or stress conditions, we demonstrated for the first time that, under serum deprivation, NGF sustained the activity of some key enzymes involved in energy metabolism. Moreover, we confirmed that NGF promotes myogenic fusion and expression of the structural protein myosin heavy chain while modulating NFκB activation and the content of sHSPs correlated with the differentiation process. We conclude that p75NTR is sufficient to mediate the modulation of L6C5 myogenic differentiation by NGF in term of structural, metabolic and functional changes.
Assuntos
Metabolismo Energético/efeitos dos fármacos; Fibras Musculares Esqueléticas/efeitos dos fármacos; Mioblastos/efeitos dos fármacos; NF-kappa B/genética; Fator de Crescimento Neural/farmacologia; Receptores de Fator de Crescimento Neural/genética; 3-Hidroxiacil-CoA Desidrogenase/genética; 3-Hidroxiacil-CoA Desidrogenase/metabolismo; Animais; Ciclo Celular/efeitos dos fármacos; Ciclo Celular/genética; Diferenciação Celular/efeitos dos fármacos; Fusão Celular; Linhagem Celular; Citrato (si)-Sintase/genética; Citrato (si)-Sintase/metabolismo; Cristalinas/genética; Cristalinas/metabolismo; Meios de Cultura Livres de Soro/farmacologia; Metabolismo Energético/genética; Regulação da Expressão Gênica; Proteínas de Choque Térmico HSP27/genética; Proteínas de Choque Térmico HSP27/metabolismo; L-Lactato Desidrogenase/genética; L-Lactato Desidrogenase/metabolismo; Proteínas Associadas aos Microtúbulos/genética; Proteínas Associadas aos Microtúbulos/metabolismo; Desenvolvimento Muscular/efeitos dos fármacos; Desenvolvimento Muscular/genética; Fibras Musculares Esqueléticas/citologia; Fibras Musculares Esqueléticas/metabolismo; Mioblastos/citologia; Mioblastos/metabolismo; Cadeias Pesadas de Miosina/genética; Cadeias Pesadas de Miosina/metabolismo; NF-kappa B/metabolismo; Fator de Crescimento Neural/metabolismo; Proteínas do Tecido Nervoso; Ratos; Receptores de Fatores de Crescimento; Receptores de Fator de Crescimento Neural/metabolismo; Transdução de Sinais
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: NF-kappa B / Receptores de Fator de Crescimento Neural / Fibras Musculares Esqueléticas / Fator de Crescimento Neural / Mioblastos / Metabolismo Energético Idioma: En Revista: BMC Res Notes Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Itália
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: NF-kappa B / Receptores de Fator de Crescimento Neural / Fibras Musculares Esqueléticas / Fator de Crescimento Neural / Mioblastos / Metabolismo Energético Idioma: En Revista: BMC Res Notes Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Itália