Remarkably efficient production of a highly insecticidal Chrysodeixis chalcites nucleopolyhedrovirus (ChchNPV) isolate in its homologous host.
Pest Manag Sci
; 74(7): 1586-1592, 2018 Jul.
Article
em En
| MEDLINE
| ID: mdl-29297971
ABSTRACT
BACKGROUND:
A Chrysodeixis chalcites nucleopolyhedrovirus from the Canary Islands (ChchNPV-TF1) has proved to be effective for control of Chrysodeixis chalcites on banana crops. Commercialization of this virus as a bioinsecticide requires an efficient production system.RESULTS:
The sixth instar (L6 ) was the most suitable for virus production, producing 1.80 × 1011 occlusion bodies (OB)/larva and showed a lower prevalence of cannibalism (5.4%) than fourth (L4 ) or fifth (L5 ) instars. Inoculation of L6 at 24 h post molting produced six times more OB (5.72 × 1011 OB/larva) than recently molted L6 larvae (1.00 × 1011 OB/larva). No significant differences were recorded in mean time to death (165-175 h) or OB production per larva (3.75 × 1011 to 5.97 × 1011 ) or per mg larval weight (1.30 × 1011 to 2.11 × 109 ), in larvae inoculated with a range of inoculum concentrations (LC50 -LC90 ). Groups of infected L6 larvae reared at a density of 150 larvae/container produced a greater total number of OBs (8.07 × 1013 OB/container) than lower densities (25, 50 and 100 OB/container), and a similar number to containers with 200 inoculated larvae (8.43 × 1013 OB/container).CONCLUSION:
The processes described here allow efficient production of sufficient OBs to treat â¼ 40 ha of banana crops using the insects from a single container. © 2018 Society of Chemical Industry.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Contexto em Saúde:
2_ODS3
Problema de saúde:
2_enfermedades_transmissibles
Assunto principal:
Controle Biológico de Vetores
/
Nucleopoliedrovírus
/
Mariposas
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
País/Região como assunto:
Europa
Idioma:
En
Revista:
Pest Manag Sci
Assunto da revista:
TOXICOLOGIA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Espanha