Orthogonal, dual protein labelling by tandem cycloaddition of strained alkenes and alkynes to ortho-quinones and azides.

Bruins, Jorick J; Blanco-Ania, Daniel; van der Doef, Vincent; van Delft, Floris L; Albada, Bauke

Bruins, Jorick J; Blanco-Ania, Daniel; van der Doef, Vincent; van Delft, Floris L; Albada, Bauke.

Afiliação

Bruins JJ; Laboratory of Organic Chemistry, Wageningen University & Research, Stippeneng 4, 6708 WE, Wageningen, the Netherlands. floris.vandelft@wur.nl bauke.albada@wur.nl.
Blanco-Ania D; Institute for Molecules and Materials, Radboud University, Nijmegen, the Netherlands.
van der Doef V; Laboratory of Organic Chemistry, Wageningen University & Research, Stippeneng 4, 6708 WE, Wageningen, the Netherlands. floris.vandelft@wur.nl bauke.albada@wur.nl.
van Delft FL; Laboratory of Organic Chemistry, Wageningen University & Research, Stippeneng 4, 6708 WE, Wageningen, the Netherlands. floris.vandelft@wur.nl bauke.albada@wur.nl.
Albada B; Laboratory of Organic Chemistry, Wageningen University & Research, Stippeneng 4, 6708 WE, Wageningen, the Netherlands. floris.vandelft@wur.nl bauke.albada@wur.nl.

Chem Commun (Camb) ; 54(53): 7338-7341, 2018 Jun 28.

Article em En | MEDLINE | ID: mdl-29911239

RESUMO

Reaction of cyclopropanated trans-cyclooctene (cpTCO) with in situ generated ortho-quinone is an efficient tool for bioorthogonal protein conjugation. The (4+2)-cycloaddition of cpTCO with ortho-quinone is significantly faster than its cyclooctyne counterpart (BCN). Orthogonal, tandem cpTCO-quinone and BCN-azide cycloadditions afforded a homogeneous, dual labelled antibody-drug conjugate.

Texto completo

Adicionar na Minha BVS

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Chem Commun (Camb) Assunto da revista: QUIMICA Ano de publicação: 2018 Tipo de documento: Article

Texto completo

Adicionar na Minha BVS

Imprimir

XML

PubMed Links

Buscar no Google