Phagocytic Activation of Macrophages with Serum MAF Depends on Engulfment Efficiency and Not Migratory Activity.
Anticancer Res
; 38(7): 4295-4298, 2018 Jul.
Article
em En
| MEDLINE
| ID: mdl-29970564
ABSTRACT
BACKGROUND/AIM:
Serum-derived macrophage activating factor (serum MAF) is known to increase the phagocytic activity of macrophages and potentially plays a role in activating cancer immunity. In order to reveal the contributing factors for phagocytic activation, the migratory activity and the efficiency of engulfment was analyzed. MATERIALS ANDMETHODS:
THP-1 macrophages were induced by 12-O-tetradecanoyl-13-acetate (TPA). The migratory activity and efficiency of engulfment were analyzed by time-lapse imaging and suspension assay, respectively.RESULTS:
While the distance of migration did not change before and after activation with serum MAF, the efficiency of beads internalisation was significantly increased.CONCLUSION:
Phagocytic activation of serum-MAF-treated macrophages was caused by increasing the efficiency of engulfment. This study contributes to the knowledge about the activation of the immune system through phagocytic activation of macrophages.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fagocitose
/
Fatores Ativadores de Macrófagos
/
Ativação de Macrófagos
/
Macrófagos
Limite:
Humans
Idioma:
En
Revista:
Anticancer Res
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Japão