Large T antigen-rich viral DNA replication loci in SV40-infected monkey kidney cells.
Exp Cell Res
; 161(2): 434-44, 1985 Dec.
Article
em En
| MEDLINE
| ID: mdl-2998837
ABSTRACT
The nuclear distribution of the large T antigen (T-Ag) during lytic infection of CV1 monkey kidney cells with SV40 virus was studied by immunoelectron microscopy. The viral protein was associated with the cellular chromatin and also accumulated within a small number of clearly delimited areas of the nucleoplasm. These T-Ag-rich areas were devoid of viral particles but contain 3-10 nm DNA filaments in an amorphous matrix. We have named these areas 'viral DNA/T-Ag loci.' The combination of the immunostaining for T-Ag with ultrastructural autoradiography revealed that these viral DNA/T-Ag loci were the sites of active SV40 DNA synthesis. We suggest that the viral DNA/T-Ag loci may represent definite structural domains specifically involved in viral DNA replication regulated by SV40-T antigen.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Replicação Viral
/
DNA Viral
/
Núcleo Celular
/
Proteínas Oncogênicas Virais
/
Vírus 40 dos Símios
/
Replicação do DNA
/
Antígenos Virais de Tumores
Limite:
Animals
Idioma:
En
Revista:
Exp Cell Res
Ano de publicação:
1985
Tipo de documento:
Article