Involvement of miR-455 in the protective effect of H2S against chemical hypoxia-induced injury in BEAS-2B cells.
Pathol Res Pract
; 214(11): 1804-1810, 2018 Nov.
Article
em En
| MEDLINE
| ID: mdl-30193773
ABSTRACT
The protective effect of hydrogen sulfide (H2S) against hypoxia-induced injury via anti-apoptosis is well established, but the underlying mechanism remains unclear. The present study aimed to investigate whether miR-455 participated in the H2S protection of lung epithelial cells against CoCl2-induced apoptosis by regulating endoplasmic reticulum stress (ERS)-related genes. Human lung epithelial cells BEAS-2B were subjected to hypoxia injury with or without H2S preconditioning. It was found that hypoxia injury increased apoptosis of BEAS-2B cells, down-regulated the expression of miR-455, and upregulated the expression of calreticulin (Calr). H2S preconditioning attenuated lung epithelial cells apoptosis, enhanced cell viability, up-regulated the expression of miR-455, as well as down-regulated the expression of Calr following hypoxia injury. In addition, Calr, GRP78, C/EBP homologous protein (CHOP) and Caspase-12 protein was down-regulated by the miR-455 mimic and up-regulated by the miR-455 inhibitor. These results implicate miR-455 regulated H2S protection of lung epithelial cells against hypoxia-induced apoptosis by stimulating Calr.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Apoptose
/
MicroRNAs
/
Células Epiteliais
/
Sulfeto de Hidrogênio
/
Pulmão
Limite:
Humans
Idioma:
En
Revista:
Pathol Res Pract
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China