Single-cell Analysis with Microfluidic Devices.
Anal Sci
; 35(6): 609-618, 2019 Jun 10.
Article
em En
| MEDLINE
| ID: mdl-30853696
ABSTRACT
A microfluidic device as a pivotal research tool in chemistry and life science is now widely recognized. Indeed, microfluidic techniques have made significant advancements in fundamental research, such as the inherent heterogeneity of single-cells studies in cell populations, which would be helpful in understanding cellular molecular mechanisms and clinical diagnosis of major diseases. Single-cell analyses on microdevices have shown great potential for precise fluid control, cell manipulation, and signal output with rapid and high throughput. Moreover, miniaturized devices also have open functions such as integrating with traditional detection methods, for example, optical, electrochemical or mass spectrometry for single-cell analysis. In this review, we summarized recent advances of single-cell analysis based on various microfluidic approaches from different dimensions, such as in vitro, ex vivo, and in vivo analysis of single cells.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dispositivos Lab-On-A-Chip
/
Análise de Célula Única
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Anal Sci
Ano de publicação:
2019
Tipo de documento:
Article