Root canal dressings for revascularization influence in vitro mineralization of apical papilla cells.
J Appl Oral Sci
; 27: e20180396, 2019.
Article
em En
| MEDLINE
| ID: mdl-30994774
ABSTRACT
Endodontic revascularization is based on cell recruitment into the necrotic root canal of immature teeth after chemical disinfection. The clinical outcome depends on the ability of surviving cells from the apical tissue to differentiate and promote hard tissue deposition inside the dentinal walls. OBJECTIVE:
To investigate the effect of calcium hydroxide (CH) and modified triple antibiotic paste (mTAP - ciprofloxacin, metronidazole and cefaclor) on the viability and mineralization potential of apical papilla cells (APC) in vitro . MATERIAL ANDMETHODS:
APC cultures were kept in contact with CH or mTAP (250-1000 µg/mL) for 5 days, after which cell viability was assessed using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Next, APCs were subjected to CH or mTAP at 250 µg/mL for 5 days before inducing the differentiation assay. After 14 and 21 days, calcium deposition was assessed by the Alizarin Red S staining method, followed by elution and quantification using spectrophotometry. Data were analyzed using ANOVA followed by Tukey post hoc test.RESULTS:
CH induced cell proliferation, whereas mTAP showed significant cytotoxicity at all concentrations tested. APC treated with CH demonstrated improved mineralization capacity at 14 days, while, for mTAP, significant reduction on the mineralization rate was observed for both experimental periods (14 and 21 days).CONCLUSION:
Our findings showed that CH induces cell proliferation and improves early mineralization, whereas mTAP was found cytotoxic and reduced the mineralization potential in vitro of APCs.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Irrigantes do Canal Radicular
/
Hidróxido de Cálcio
/
Papila Dentária
/
Antibacterianos
Tipo de estudo:
Evaluation_studies
Limite:
Humans
Idioma:
En
Revista:
J Appl Oral Sci
Assunto da revista:
ODONTOLOGIA
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Brasil