A complex human gut microbiome cultured in an anaerobic intestine-on-a-chip.
Nat Biomed Eng
; 3(7): 520-531, 2019 07.
Article
em En
| MEDLINE
| ID: mdl-31086325
ABSTRACT
The diverse bacterial populations that comprise the commensal microbiome of the human intestine play a central role in health and disease. A method that sustains complex microbial communities in direct contact with living human intestinal cells and their overlying mucus layer in vitro would thus enable the investigation of host-microbiome interactions. Here, we show the extended coculture of living human intestinal epithelium with stable communities of aerobic and anaerobic human gut microbiota, using a microfluidic intestine-on-a-chip that permits the control and real-time assessment of physiologically relevant oxygen gradients. When compared to aerobic coculture conditions, the establishment of a transluminal hypoxia gradient in the chip increased intestinal barrier function and sustained a physiologically relevant level of microbial diversity, consisting of over 200 unique operational taxonomic units from 11 different genera and an abundance of obligate anaerobic bacteria, with ratios of Firmicutes and Bacteroidetes similar to those observed in human faeces. The intestine-on-a-chip may serve as a discovery tool for the development of microbiome-related therapeutics, probiotics and nutraceuticals.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Contexto em Saúde:
3_ND
Problema de saúde:
3_zoonosis
Assunto principal:
Técnicas de Cultura de Células
/
Técnicas Analíticas Microfluídicas
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Dispositivos Lab-On-A-Chip
/
Microbiota
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Microbioma Gastrointestinal
/
Mucosa Intestinal
Limite:
Humans
Idioma:
En
Revista:
Nat Biomed Eng
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Estados Unidos