Subcellular localization of a PhoE-LacZ fusion protein in E. coli by protease accessibility experiments reveals an inner-membrane-spanning form of the protein.
FEBS Lett
; 221(2): 226-30, 1987 Sep 14.
Article
em En
| MEDLINE
| ID: mdl-3114001
ABSTRACT
Protease accessibility experiments were employed to localize a PhoE-LacZ hybrid protein, encompassing a large N-terminal fragment of the outer membrane PhoE protein of E. coli, fused to beta-galactosidase, at the subcellular level. In previous studies, this protein was shown to co-fractionate with the outer membrane, whereas immunocytochemical methods suggested a cytoplasmic location. The present results confirm the latter localization. Moreover, it appears that a minor amount of hybrid protein spans the inner membrane, with the PhoE moiety in the periplasm and the beta-galactosidase moiety in the cytoplasm. These membrane-spanning proteins might be responsible for the lethal jamming of the export machinery, observed upon induction of synthesis of the protein.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Contexto em Saúde:
3_ND
Problema de saúde:
3_neglected_diseases
/
3_zoonosis
Assunto principal:
Proteínas da Membrana Bacteriana Externa
/
Proteínas Recombinantes de Fusão
/
Proteínas Recombinantes
/
Beta-Galactosidase
/
Escherichia coli
/
Galactosidases
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1987
Tipo de documento:
Article