[Effect of Quercetin on Apoptosis of Platelets and Its Mechanism].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
; 27(5): 1612-1616, 2019 Oct.
Article
em Zh
| MEDLINE
| ID: mdl-31607321
ABSTRACT
OBJECTIVE:
To investigate the effects of quercetin on the apoptosis of platelets and to analyze the intrinsic mechanism.METHODS:
Firstly, the effects of quecetin on the apoptosis of platelets was detected by flow cytometry. Secondly, Western blot was used to detect the expression of apoptosis-related proteins in the platelets treated with quercetin for 2 and 4 day.RESULTS:
By flow cytometry, it was found that the apoptosis of platelets in the quercetin-treated group (2, 4 and 8 µmol/L) was inhibited, the apoptosis rate of platelets in 2, 4 and 8 µmol/L quercetin group was 3.12%±0.32%, 2.89%±0.15% and 2.31%±0.28%, respectively, which were signigicantly lover than that in control group (Pï¼0.01). With the increase of quecetin concentration, the proportion ratio of platelets significantly decreased in a concentration-dependent mannerï¼r=-0.9985ï¼. Similar results were observed on the 4th day. Western blot showed that the treatment with quercetin (2, 4 and 8 µmol/L) promoted the expression of anti-apoptotic protein BCL-2, inhibited the expression of pro-apoptotic protein BAX, resulting in a significant increase in the ratio of BCL-2/BAX (Pï¼0.01), thereby inhibiting the apoptosis of platelets. Similar results were observed on the 4th day.CONCLUSION:
Quercetin can inhibit platelet apoptosis by increasing the ratio of apoptosis-related protein BCL-2/BAX in a concentration-dependent manner.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plaquetas
Idioma:
Zh
Revista:
Zhongguo Shi Yan Xue Ye Xue Za Zhi
Assunto da revista:
HEMATOLOGIA
Ano de publicação:
2019
Tipo de documento:
Article