The Pseudomonas aeruginosa HSP70-like protein DnaK induces IL-1ß expression via TLR4-dependent activation of the NF-κB and JNK signaling pathways.
Comp Immunol Microbiol Infect Dis
; 67: 101373, 2019 Dec.
Article
em En
| MEDLINE
| ID: mdl-31704499
ABSTRACT
IL-1ß expression is increased in response to P. aeruginosa infection, but the responsible proteins have not been clearly elucidated. Here, we demonstrate for the first time that IL-1ß expression is induced in response to the heat shock protein 70-like protein DnaK. Treatment with recombinant DnaK (rDnaK) increased IL-1ßâ¯expression in a dose- and time-dependent manner, and the release of mature IL-1ß in response to rDnaK was detected to an extent similar to that stimulated by the well-known agonists, lipopolysaccharide and nigericin. rDnaK-mediated IL-1ß expression was driven by the NF-κB signaling pathway. In addition, expression was controlled by the JNK signaling pathway, although these two signaling cascades act independently upon rDnaK stimulation. Finally, rDnaK-induced IL-1ß expression was initiated via the action of TLR4. Taken together, the data reveal that P. aeruginosa-derived DnaK induces expression of IL-1ß via TLR4-dependent activation of the NF-κB and JNK signaling pathways.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pseudomonas aeruginosa
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NF-kappa B
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Proteínas de Choque Térmico HSP70
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Proteínas Quinases JNK Ativadas por Mitógeno
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Receptor 4 Toll-Like
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Interleucina-1beta
Limite:
Humans
Idioma:
En
Revista:
Comp Immunol Microbiol Infect Dis
Ano de publicação:
2019
Tipo de documento:
Article