Immobilization of Thermoalkalophilic Lipase from Bacillus atrophaeus FSHM2 on Amine-Modified Graphene Oxide Nanostructures: Statistical Optimization and Its Application for Pentyl Valerate Synthesis.
Appl Biochem Biotechnol
; 191(2): 579-604, 2020 Jun.
Article
em En
| MEDLINE
| ID: mdl-31823273
ABSTRACT
Synthesis of (3-aminopropyl) triethoxysilane (APTES)-functionalized graphene oxide (GO) nanosheets, statistical optimization of conditions for immobilization of Bacillus atrophaeus lipase (BaL) on as-synthesized support, and application of the immobilized BaL for esterification of valeric acid were carried out in this investigation. The optimum specific activity of the immobilized BaL (81.60 ± 0.28 U mg-1) was achieved at 3 mg mL-1 of GO-NH2, 50 mM of phosphate buffer, pH 7.0, 60 min sonication time, 100 mM glutaraldehyde, 25 U mL-1 of enzyme, and 8 h immobilization time at 4 °C. The immobilized BaL retained about 90% of its initial activity after 10 days of storage. Moreover, about 70% of the initial activity of the immobilized BaL was retained after 10 cycles of application. The results of esterification studies exhibited that maximum pentyl valerate synthesis using the free BaL (34.5%) and the immobilized BaL (92.7%) occurred in the organic solvent medium (xylene) after 48 h of incubation at 60 °C.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bacillus
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Valeratos
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Nanoestruturas
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Enzimas Imobilizadas
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Aminas
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Grafite
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Lipase
Idioma:
En
Revista:
Appl Biochem Biotechnol
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Irã