Molecular detection of reptile-associated Borrelia in Boa constrictor (Squamata: Boidae) from Veracruz, Mexico.
Acta Trop
; 205: 105422, 2020 May.
Article
em En
| MEDLINE
| ID: mdl-32112720
ABSTRACT
The reptile-associated Borrelia represent a monophyletic group of bacteria transmitted by several species of hard ticks, which has been reported to only infect amphibians and reptiles in Eurasia and Middle East, however, this bacterial group has not been studied in North America. The aim of this study was to assess the presence of Borrelia spirochetes in blood samples of native reptiles of Mexico. Blood samples were directly obtained from individuals, DNA extractions were performed using Chelex-100. The Borrelia detection was performed by conventional PCR. From 102 reptiles tested, only five individuals of Boa constrictor were positive for the presence of DNA of the reptile-associated Borrelia group. Supported by phylogenetic analysis, this study presents the first record of these spirochetes group in Mexico, and initial evidence of B. constrictor as a host of this group.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Borrelia
/
Infecções por Borrelia
/
Boidae
Tipo de estudo:
Diagnostic_studies
/
Risk_factors_studies
Limite:
Animals
País/Região como assunto:
Mexico
Idioma:
En
Revista:
Acta Trop
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
México