Transcriptome analysis of Liriomyza trifolii (Diptera: Agromyzidae) in response to temperature stress.
Comp Biochem Physiol Part D Genomics Proteomics
; 34: 100677, 2020 06.
Article
em En
| MEDLINE
| ID: mdl-32143024
ABSTRACT
The leafminer Liriomyza trifolii is an important insect pest of ornamental and vegetable crops worldwide. Temperature is a critical environmental factor that impacts both the distribution and interspecific competition of Liriomyza spp. In this study, we compared the transcriptomes of L. trifolii exposed to ambient (25 °C), hot (43 °C), and cold (-7 °C) temperatures. RNA-seq revealed 100,041 assembled unigenes, and 50,546 of these were annotated in L. trifolii transcriptome libraries. A total of 207 and 2904 differentially expressed genes (DEGs) were identified in response to hot and cold stress, respectively. Functional classification indicated that "cellular process", "single organism processes" and "metabolic processes" pathways were significantly enriched, along with "binding activity" and "catalytic activity". With respect to clusters of orthologous genes (COG) classification, DEGs were assigned to "post-translational modification, protein turnover, chaperones", "carbohydrate transport and metabolism" and "lipid transport and metabolism" categories. Subsequent annotation and enrichment analyses indicated that genes encoding heat shock proteins (HSPs) and cuticular proteins were significantly up-regulated during heat and cold stress, respectively. This study expands our knowledge of gene expression in L. trifolii during temperature stress and provides a basis for further studies aimed at understanding the mechanism of thermotolerance in this important invasive leafminer fly.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Estresse Fisiológico
/
Temperatura
/
Proteínas de Insetos
/
Dípteros
/
Transcriptoma
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Comp Biochem Physiol Part D Genomics Proteomics
Assunto da revista:
BIOLOGIA
/
GENETICA
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China