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Targeting lentiviral vectors to primordial germ cells (PGCs): An efficient strategy for generating transgenic chickens.
Jiang, Zi-Qin; Wu, Han-Yu; Tian, Jing; Li, Ning; Hu, Xiao-Xiang.
Afiliação
  • Jiang ZQ; College of Biological Sciences, China Agricultural University, Beijing 100094, China.
  • Wu HY; State Key Laboratory of Agro-Biotechnology, China Agricultural University, Beijing 100094, China.
  • Tian J; College of Biological Sciences, China Agricultural University, Beijing 100094, China.
  • Li N; State Key Laboratory of Agro-Biotechnology, China Agricultural University, Beijing 100094, China.
  • Hu XX; College of Biological Sciences, China Agricultural University, Beijing 100094, China.
Zool Res ; 41(3): 281-291, 2020 May 18.
Article em En | MEDLINE | ID: mdl-32274905
ABSTRACT
Recent advances in avian transgenic studies highlight the possibility of utilizing lentiviral vectors as tools to generate transgenic chickens. However, low rates of gonadal chimerism and germ line transmission efficiency still limit the broad usage of this method in creating transgenic chickens. In this study, we implemented a simple strategy using modified lentiviral vectors targeted to chicken primordial germ cells (PGCs) to generate transgenic chickens. The lentiviral vectors were pseudotyped with a modified Sindbis virus envelope protein (termed M168) and conjugated with an antibody specific to PGC membrane proteins. We demonstrated that these optimized M168-pseudotyped lentiviral vectors conjugated with SSEA4 antibodies successfully targeted transduction of PGCs in vitro and in vivo. Compared with the control, 50.0%-66.7% of chicken embryos expressed green fluorescent protein (GFP) in gonads transduced by the M168-pseudotyped lentivirus. This improved the targeted transduction efficiency by 30.0%-46.7%. Efficient chimerism of exogenous genes was also observed. This targeting technology could improve the efficiency of germ line transmission and provide greater opportunities for transgenic poultry studies.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Animais Geneticamente Modificados / Galinhas / Lentivirus / Vetores Genéticos / Células Germinativas Limite: Animals Idioma: En Revista: Zool Res Ano de publicação: 2020 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Animais Geneticamente Modificados / Galinhas / Lentivirus / Vetores Genéticos / Células Germinativas Limite: Animals Idioma: En Revista: Zool Res Ano de publicação: 2020 Tipo de documento: Article País de afiliação: China
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