Role of MicroRNAs in regulation of DNA damage in monocytes exposed to polystyrene and TiO2 nanoparticles.

ABSTRACT

The release of nanoparticles into the environment can interfere with the health of the exposed organisms. MicroRNAs have been suggested as potential toxicology biomarkers. The expression of potential zebrafish nano-toxicity biomarker miRNAs in our previous study was validated in THP-1 human monocytic cell line after exposure to polystyrene (PSNPs) and ARS labeled Titanium dioxide nanoparticles (nano-TiO2-ARS). miRNAs expression post exposure to PLGA nanoparticles and E. coli BioParticles was used to exclude potential activation and engagement of miRNAs through phagocytosis or pro-inflammatory specific responses. miR-155-5p showed the highest potential to be used as biomarker for PSNPs and nano-TiO2-ARS induced toxicity. To determine effects of PSNPs and nano-TiO2-ARS on genotoxicity, time and dose dependent DNA damage profile was established. Severe DNA damage was triggered by both nanoparticles, and expression of DNA damage repairing genes was elevated post nano-TiO2-ARS, but not post PSNPs exposure, questioning the utility of the comet assay as universal assessment tool for genotoxicity induced by nanoparticles in general. Transfection of miR-155-5p mimic influenced the expression of miR-155-5p related, DNA damage responsible genes post both nano-TiO2-ARS and PSNPs exposure. Transfection results suggest significant involvement of miR-155-5p in gene repair mechanisms triggered by adverse effects of PSNPs and nano-TiO2-ARS on monocytes.