T-Cell Epitope Immunotherapy in Mouse Models of Food Allergy.
Methods Mol Biol
; 2223: 337-355, 2021.
Article
em En
| MEDLINE
| ID: mdl-33226603
ABSTRACT
Food allergy has been rising in prevalence over the last two decades, affecting more than 10% of the world population. Current management of IgE-mediated food allergy relies on avoidance and rescue medications; research into treatments that are safer and providing guaranteed and durable curative effects is, therefore, essential. T-cell epitope-based immunotherapy holds the potential for modulating food allergic responses without IgE cross-linking. In this chapter, we describe the methods in evaluating the therapeutic capacities of immunodominant T-cell epitopes in animal models of food allergy. Moreover, we explain in detail the methods to measure the allergen-specific antibody levels, prepare single-cell suspension from spleen, and prepare small intestine for immunohistochemical analysis of eosinophils and Foxp3+ cells.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Contexto em Saúde:
3_ND
Problema de saúde:
3_cholera
/
3_zoonosis
Assunto principal:
Peptídeos
/
Alérgenos
/
Hipersensibilidade a Leite
/
Dessensibilização Imunológica
/
Hipersensibilidade a Ovo
/
Modelos Animais de Doenças
/
Hipersensibilidade a Frutos do Mar
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
China