Molecular insights into meningococcal carriage isolates from Burkina Faso 7 years after introduction of a serogroup A meningococcal conjugate vaccine.
Microb Genom
; 6(12)2020 12.
Article
em En
| MEDLINE
| ID: mdl-33332261
ABSTRACT
In 2010, Burkina Faso completed the first nationwide mass-vaccination campaign of a meningococcal A conjugate vaccine, drastically reducing the incidence of disease caused by serogroup A meningococci. Since then, other strains, such as those belonging to serogroups W, X and C, have continued to cause outbreaks within the region. A carriage study was conducted in 2016 and 2017 in the country to characterize the meningococcal strains circulating among healthy individuals following the mass-vaccination campaign. Four cross-sectional carriage evaluation rounds were conducted in two districts of Burkina Faso, Kaya and Ouahigouya. Oropharyngeal swabs were collected for the detection of Neisseria meningitidis by culture. Confirmed N. meningitidis isolates underwent whole-genome sequencing for molecular characterization. Among 13â758 participants, 1035 (7.5â%) N. meningitidis isolates were recovered. Most isolates (934/1035; 90.2â%) were non-groupable and primarily belonged to clonal complex (CC) 192 (822/934; 88â%). Groupable isolates (101/1035; 9.8â%) primarily belonged to CCs associated with recent outbreaks in the region, such as CC11 (serogroup W) and CC10217 (serogroup C); carried serogroup A isolates were not detected. Phylogenetic analysis revealed several CC11 strains circulating within the country, several of which were closely related to invasive isolates. Three sequence types (STs) were identified among eleven CC10217 carriage isolates, two of which have caused recent outbreaks in the region (ST-10217 and ST-12446). Our results show the importance of carriage studies to track the outbreak-associated strains circulating within the population in order to inform future vaccination strategies and molecular surveillance programmes.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Contexto em Saúde:
1_ASSA2030
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2_ODS3
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3_ND
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4_TD
Problema de saúde:
1_doencas_nao_transmissiveis
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1_surtos_doencas_emergencias
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2_enfermedades_transmissibles
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3_zoonosis
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4_meningitis
Assunto principal:
Portador Sadio
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Vacinas Meningocócicas
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Sequenciamento Completo do Genoma
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Meningite Meningocócica
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Neisseria meningitidis
Tipo de estudo:
Clinical_trials
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Observational_studies
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Prevalence_studies
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Risk_factors_studies
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Screening_studies
Limite:
Female
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Humans
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Male
País/Região como assunto:
Africa
Idioma:
En
Revista:
Microb Genom
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Estados Unidos