Simultaneous and ultrasensitive detection of three pesticides using a surface-enhanced Raman scattering-based lateral flow assay test strip.
Biosens Bioelectron
; 181: 113149, 2021 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-33713951
ABSTRACT
Chlorothalonil (CHL), imidacloprid (IMI) and oxyfluorfen (OXY) are commonly used in combination to increase crop yield. However, these three pesticides are toxic to aquatic organisms and do not easily degrade. In this study, a surface-enhanced Raman scattering-based lateral flow assay (SERS-LFA) test strip was prepared by combining antibodies with SERS nanotags, and then competitive immune binding was used to detect the three pesticides simultaneously. Moreover, the two-way binding effect of ssDNA-streptavidin bound to Ag4-NTP@AuNPs and Ag4-NTP@AuNPs with antibodies was used to further amplify the detection signal. Under the optimal conditions, the SERS-LFA test strips exhibited high sensitivity, a low detection limit, short detection time, high specificity and low cost. Furthermore, the detection range was within the values prescribed by international detection standards. By measuring the intensity of the SERS signal on the test line of the paper strip, accurate quantitative analysis was achieved. The practical application of the proposed system was demonstrated by simultaneous detection of CHL, IMI and OXY in environmental and food samples with satisfactory results.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Praguicidas
/
Técnicas Biossensoriais
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Nanopartículas Metálicas
Tipo de estudo:
Diagnostic_studies
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Guideline
Idioma:
En
Revista:
Biosens Bioelectron
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
2021
Tipo de documento:
Article