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Assessment of a PCL-3D Printing-Dental Pulp Stem Cells Triplet for Bone Engineering: An In Vitro Study.
Rosales-Ibáñez, Raúl; Cubo-Mateo, Nieves; Rodríguez-Navarrete, Amairany; González-González, Arely M; Villamar-Duque, Tomás E; Flores-Sánchez, Leticia O; Rodríguez-Lorenzo, Luis M.
Afiliação
  • Rosales-Ibáñez R; Tissue Engineering Lab, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México (UNAM), Av. Tenayuca-Chalmita S/N, Cuautepec Barrio Bajo, Alcaldía Gustavo A. Madero, Ciudad de México CP. 07239, Mexico.
  • Cubo-Mateo N; Sensors and Ultrasonic Systems Department, Institute for Physical and Information Technologies, (ITEFI-CSIC), C/ Serrano 144, 28006 Madrid, Spain.
  • Rodríguez-Navarrete A; Universidad Internacional de Valencia, C/ Pintor Sorolla 21, 46002 Valencia, Spain.
  • González-González AM; Tissue Engineering Lab, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México (UNAM), Av. Tenayuca-Chalmita S/N, Cuautepec Barrio Bajo, Alcaldía Gustavo A. Madero, Ciudad de México CP. 07239, Mexico.
  • Villamar-Duque TE; Tissue Engineering Lab, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México (UNAM), Av. Tenayuca-Chalmita S/N, Cuautepec Barrio Bajo, Alcaldía Gustavo A. Madero, Ciudad de México CP. 07239, Mexico.
  • Flores-Sánchez LO; Bioterio, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México (UNAM), Av. De los Barrios No.1, Tlalnepantla, Estado de México CP. 54090, Mexico.
  • Rodríguez-Lorenzo LM; Bioterio, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México (UNAM), Av. De los Barrios No.1, Tlalnepantla, Estado de México CP. 54090, Mexico.
Polymers (Basel) ; 13(7)2021 Apr 04.
Article em En | MEDLINE | ID: mdl-33916576
ABSTRACT
The search of suitable combinations of stem cells, biomaterials and scaffolds manufacturing methods have become a major focus of research for bone engineering. The aim of this study was to test the potential of dental pulp stem cells to attach, proliferate, mineralize and differentiate on 3D printed polycaprolactone (PCL) scaffolds. A 100% pure Mw 84,500 ± 1000 PCL was selected. 5 × 10 × 5 mm3 parallelepiped scaffolds were designed as a wood-pilled structure composed of 20 layers of 250 µm in height, in a non-alternate order ([0,0,0,90,90,90°]). 3D printing was made at 170 °C. Swine dental pulp stem cells (DPSCs) were extracted from lower lateral incisors of swine and cultivated until the cells reached 80% confluence. The third passage was used for seeding on the scaffolds. Phenotype of cells was determined by flow Cytometry. Live and dead, Alamar blue™, von Kossa and alizarin red staining assays were performed. Scaffolds with 290 + 30 µm strand diameter, 938 ± 80 µm pores in the axial direction and 689 ± 13 µm pores in the lateral direction were manufactured. Together, cell viability tests, von Kossa and Alizarin red staining indicate the ability of the printed scaffolds to support DPSCs attachment, proliferation and enable differentiation followed by mineralization. The selected material-processing technique-cell line (PCL-3D printing-DPSCs) triplet can be though to be used for further modelling and preclinical experiments in bone engineering studies.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Polymers (Basel) Ano de publicação: 2021 Tipo de documento: Article País de afiliação: México

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Polymers (Basel) Ano de publicação: 2021 Tipo de documento: Article País de afiliação: México
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