Demethyleneberberine promotes apoptosis and suppresses TGF-ß/Smads induced EMT in the colon cancer cells HCT-116.
Cell Biochem Funct
; 39(6): 763-770, 2021 Aug.
Article
em En
| MEDLINE
| ID: mdl-34028068
ABSTRACT
Colorectal cancer (CRC) is one of the most common malignant tumours in the world. Recent reports have revealed natural products displayed inhibition on colon cancer potential by suppressing transforming growth factor-ß/Smads induced epidermal-mesenchymal transition (EMT). In this article, 12 kinds of natural berberine analogues were screened for their effects on the inhibition of the colon cancer cells, the results showed that demethyleneberberine (DM-BBR) exhibited an interesting and potential effect on inducing the apoptosis of HCT-116 cells with drug concentrations of 6, 12 and 18 µM. Particularly, DM-BBR reversed the EMT process by inhibiting the expression of p-Smad2 and p-Smad3 in the transforming growth factor-ß/Smads signal pathway, up-regulated pro-apoptotic protein cleaved caspase-9, and blocked cell cycle at the S phase and increasing the expression of cyclin proteins P27 and P21. Taken together, these findings suggested that DM-BBR could promote apoptosis and suppress TGF-ß/Smads induced EMT in the colon cancer cells HCT-116.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Berberina
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Fator de Crescimento Transformador beta
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Neoplasias do Colo
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Proteína Smad2
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Proteína Smad3
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Transição Epitelial-Mesenquimal
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Antineoplásicos
Limite:
Humans
Idioma:
En
Revista:
Cell Biochem Funct
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
China