Luciferase reporter assays to monitor interferon signaling modulation by SARS-CoV-2 proteins.
STAR Protoc
; 2(4): 100781, 2021 12 17.
Article
em En
| MEDLINE
| ID: mdl-34405154
ABSTRACT
We present a protocol for analyzing the impact of SARS-CoV-2 proteins in interferon signaling using luciferase reporter assays. Here, the induction of defined promoters can be quantitatively assessed with high sensitivity and broad linear range. The results are similar to those obtained using qPCR to measure endogenous mRNA induction. The assay requires stringent normalization and confirmation of the results in more physiological settings. The protocol is adaptable for other viruses and other innate immune stimuli. For complete details on the use and execution of this protocol, please refer to Hayn et al. (2021).
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Virais
/
RNA Mensageiro
/
Regulação Viral da Expressão Gênica
/
Interferons
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SARS-CoV-2
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COVID-19
/
Luciferases
Limite:
Humans
Idioma:
En
Revista:
STAR Protoc
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Alemanha