Detection of Mouse Type I NKT (iNKT) Cells by Flow Cytometry.
Methods Mol Biol
; 2388: 87-99, 2021.
Article
em En
| MEDLINE
| ID: mdl-34524664
ABSTRACT
Flow cytometry is an effective tool in immunology that uses laser as a light source to yield scattered and fluorescent light signals read by photomultiplier tubes or photodiodes for detection. Flow cytometry allows immunophenotyping using fluorescently conjugated antibodies for the identification of subgroups of immune cells at a single-cell level. Natural killer T (NKT) cells are CD1d-restricted T cells, which recognize lipid antigens, unlike conventional T lymphocytes that recognize peptide antigens presented by class I or class II MHC. The unique T cell receptor (TCR) of type I NKT or invariant natural killer T (iNKT) cells are comprised of an invariant α-chain that pairs with a limited repertoire of ß-chains. Type I NKT cells play an essential role in the orchestration of the innate and adaptive immune responses against various diseases. Here, we will review the process of identifying mouse type I NKT cells by flow cytometry, which serves as a foundational technique for studying these cells.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células T Matadoras Naturais
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Estados Unidos