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3D-cardiomics: A spatial transcriptional atlas of the mammalian heart.
Mohenska, Monika; Tan, Nathalia M; Tokolyi, Alex; Furtado, Milena B; Costa, Mauro W; Perry, Andrew J; Hatwell-Humble, Jessica; van Duijvenboden, Karel; Nim, Hieu T; Ji, Yuan M M; Charitakis, Natalie; Bienroth, Denis; Bolk, Francesca; Vivien, Celine; Knaupp, Anja S; Powell, David R; Elliott, David A; Porrello, Enzo R; Nilsson, Susan K; Del Monte-Nieto, Gonzalo; Rosenthal, Nadia A; Rossello, Fernando J; Polo, Jose M; Ramialison, Mirana.
Afiliação
  • Mohenska M; Department of Anatomy and Developmental Biology, Monash University, Wellington Road, Clayton, Victoria, Australia; Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Wellington Road, Clayton, Victoria, Australia; Australian Regenerative Medicine Institute, Monash University,
  • Tan NM; Department of Anatomy and Developmental Biology, Monash University, Wellington Road, Clayton, Victoria, Australia; Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Wellington Road, Clayton, Victoria, Australia; Australian Regenerative Medicine Institute, Monash University,
  • Tokolyi A; Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, Victoria, Australia.
  • Furtado MB; Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, Victoria, Australia; The Jackson Laboratory, Bar Harbor, ME, USA.
  • Costa MW; Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, Victoria, Australia; The Jackson Laboratory, Bar Harbor, ME, USA.
  • Perry AJ; Monash Bioinformatics Platform, Monash University, Wellington Road, Clayton, Victoria, Australia.
  • Hatwell-Humble J; Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, Victoria, Australia; Biomedical Manufacturing, CSIRO Manufacturing, Bag 10, Clayton South, Australia.
  • van Duijvenboden K; Department of Medical Biology, Academic Medical Centre, Amsterdam, the Netherlands.
  • Nim HT; Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, Victoria, Australia; Faculty of Information Technology, Monash University, Clayton, Victoria, Australia; Murdoch Children's Research Institute, Royal Children's Hospital, Melbourne 3052, VIC, Australia; Systems
  • Ji YMM; Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, Victoria, Australia.
  • Charitakis N; Murdoch Children's Research Institute, Royal Children's Hospital, Melbourne 3052, VIC, Australia; Department of Paediatrics, University of Melbourne, Parkville, VIC, Australia.
  • Bienroth D; Murdoch Children's Research Institute, Royal Children's Hospital, Melbourne 3052, VIC, Australia.
  • Bolk F; Murdoch Children's Research Institute, Royal Children's Hospital, Melbourne 3052, VIC, Australia; Melbourne Centre for Cardiovascular Genomics and Regenerative Medicine, The Royal Children's Hospital, Melbourne 3052, VIC, Australia.
  • Vivien C; Murdoch Children's Research Institute, Royal Children's Hospital, Melbourne 3052, VIC, Australia.
  • Knaupp AS; Department of Anatomy and Developmental Biology, Monash University, Wellington Road, Clayton, Victoria, Australia; Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Wellington Road, Clayton, Victoria, Australia; Australian Regenerative Medicine Institute, Monash University,
  • Powell DR; Monash Bioinformatics Platform, Monash University, Wellington Road, Clayton, Victoria, Australia.
  • Elliott DA; Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, Victoria, Australia; Murdoch Children's Research Institute, Royal Children's Hospital, Melbourne 3052, VIC, Australia; Department of Paediatrics, University of Melbourne, Parkville, VIC, Australia.
  • Porrello ER; Murdoch Children's Research Institute, Royal Children's Hospital, Melbourne 3052, VIC, Australia; Melbourne Centre for Cardiovascular Genomics and Regenerative Medicine, The Royal Children's Hospital, Melbourne 3052, VIC, Australia; Department of Anatomy and Physiology, School of Biomedical Sciences
  • Nilsson SK; Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, Victoria, Australia; Biomedical Manufacturing, CSIRO Manufacturing, Bag 10, Clayton South, Australia.
  • Del Monte-Nieto G; Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, Victoria, Australia.
  • Rosenthal NA; Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, Victoria, Australia; The Jackson Laboratory, Bar Harbor, ME, USA; National Heart and Lung Institute, Imperial College London, London, United Kingdom.
  • Rossello FJ; Department of Anatomy and Developmental Biology, Monash University, Wellington Road, Clayton, Victoria, Australia; Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Wellington Road, Clayton, Victoria, Australia; Australian Regenerative Medicine Institute, Monash University,
  • Polo JM; Department of Anatomy and Developmental Biology, Monash University, Wellington Road, Clayton, Victoria, Australia; Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Wellington Road, Clayton, Victoria, Australia; Australian Regenerative Medicine Institute, Monash University,
  • Ramialison M; Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, Victoria, Australia; The Jackson Laboratory, Bar Harbor, ME, USA; Murdoch Children's Research Institute, Royal Children's Hospital, Melbourne 3052, VIC, Australia; Systems Biology Institute Australia, Clayton, V
J Mol Cell Cardiol ; 163: 20-32, 2022 02.
Article em En | MEDLINE | ID: mdl-34624332
ABSTRACT
Understanding the spatial gene expression and regulation in the heart is key to uncovering its developmental and physiological processes, during homeostasis and disease. Numerous techniques exist to gain gene expression and regulation information in organs such as the heart, but few utilize intuitive true-to-life three-dimensional representations to analyze and visualise results. Here we combined transcriptomics with 3D-modelling to interrogate spatial gene expression in the mammalian heart. For this, we microdissected and sequenced transcriptome-wide 18 anatomical sections of the adult mouse heart. Our study has unveiled known and novel genes that display complex spatial expression in the heart sub-compartments. We have also created 3D-cardiomics, an interface for spatial transcriptome analysis and visualization that allows the easy exploration of these data in a 3D model of the heart. 3D-cardiomics is accessible from http//3d-cardiomics.erc.monash.edu/.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transcriptoma / Coração Limite: Animals Idioma: En Revista: J Mol Cell Cardiol Ano de publicação: 2022 Tipo de documento: Article
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transcriptoma / Coração Limite: Animals Idioma: En Revista: J Mol Cell Cardiol Ano de publicação: 2022 Tipo de documento: Article