Observation of targeted gold nanoparticles in nasopharyngeal tumour nude mice model through dual-energy computed tomography.

ABSTRACT

This study was performed to specify the efficiency of imaging nanoparticle concentration as contrast media in dual-energy computed tomography (DECT). Gold nanoparticles (AuNPs) and gold nanoparticles-conjugated folic acid through cysteamine (FA-Cya-AuNPs) were both considered as contrast agents. Characterization of NPs was performed using Dynamic Light Scattering (DLS) and zeta potential. The hemocompatibility of NPs was confirmed by different blood parameters such as white blood cell, red cell distribution width, hemoglobin, lymphocytes counts and haemolysis assay. DECT algorithm was confirmed using calibration phantom at different concentrations of NPs and tube potentials (80 and 140 kVp). Then, DECT was used to quantify the concentration of both AuNPs and FA-Cys-AuNPs in human nasopharyngeal cancer cells. Mice were injected with non-targeted AuNPs and targeted AuNps at a concentration of 3 × 103 µg/ml. Then, they were scanned with different tube potentials. The concentration of nanoparticles in the various organs of nude mice was measured through DECT imaging and inductively coupled plasma mass spectrometry (ICP-MS) analysis. The results of DECT images were compared with ICP-MS analysis and indicated that they were approximately similar. In sum, FA-Cys-AuNPs can be a proper candidate for targeted contrast media in DECT molecular scanning of human nasopharyngeal tumours.