Antigenicity and immunogenicity of HA2 and M2e influenza virus antigens conjugated to norovirus-like, VP1 capsid-based particles by the SpyTag/SpyCatcher technology.
Virology
; 566: 89-97, 2022 01.
Article
em En
| MEDLINE
| ID: mdl-34894525
ABSTRACT
Virus-like particles (VLPs) modified through different molecular technologies are employed as delivery vehicles or platforms for heterologous antigen display. We have recently created a norovirus (NoV) VLP platform, where two influenza antigens, the extracellular domain of matrix protein M2 (M2e) or the stem domain of the major envelope glycoprotein hemagglutinin (HA2) are displayed on the surface of the NoV VLPs by SpyTag/SpyCatcher conjugation. To demonstrate the feasibility of the platform to deliver foreign antigens, this study examined potential interference of the conjugation with induction of antibodies against conjugated M2e peptide, HA2, and NoV VLP carrier. High antibody response was induced by HA2 but not M2e decorated VLPs. Furthermore, HA2-elicited antibodies did not neutralize the homologous influenza virus in vitro. Conjugated NoV VLPs retained intact receptor binding capacity and self-immunogenicity. The results demonstrate that NoV VLPs could be simultaneously used as a platform to deliver foreign antigens and a NoV vaccine.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Contexto em Saúde:
1_ASSA2030
/
2_ODS3
Problema de saúde:
1_doencas_transmissiveis
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2_enfermedades_transmissibles
Assunto principal:
Imunoglobulina G
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Vacinas contra Influenza
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Infecções por Orthomyxoviridae
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Influenza Humana
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Vacinas de Partículas Semelhantes a Vírus
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Hemaglutininas Virais
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Anticorpos Antivirais
Limite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Virology
Ano de publicação:
2022
Tipo de documento:
Article