H65 fusion protein fails to improve the protection of a rationally attenuated live vaccine candidate against bovine tuberculosis in a mouse model of tuberculosis.
Int J Mycobacteriol
; 10(4): 411-413, 2021.
Article
em En
| MEDLINE
| ID: mdl-34916460
ABSTRACT
Background:
The fusion protein H65, composed of Mycobacterium tuberculosis (TB) ESX-secreted antigens, has improved the bacillus Calmette-Guerin-induced immune protection in a mouse model of bovine TB when formulated in the liposomal adjuvant CAF01. In this study, we aimed to evaluate the protective efficacy of an attenuated Mycobacterium bovis strain - a mutant in mce2 and phoP genes - combined with H65+CAF01 immunization. We evaluated the protection of MbΔmce2-phoP alone or combined with H65+CAF01 against M. bovis challenge in mice.Methods:
Groups of BALBc mice were inoculated with the vaccine candidates or phosphate buffered saline (PBS), and 6 weeks after the last immunization, the animals were aerogenically challenged with virulent M. bovis. Bacterial load in organs was counted after 45 days of the challenge. One-way analysis of variance and Bonferroni's posttest were used for statistical analysis.Results:
All vaccinated mice showed reduced bacterial loads in lungs compared to unvaccinated animals. However, the protection level was similar between vaccinated groups.Conclusions:
The MbΔmce2-phoP strain combined with three doses of H65+CAF01 induced equivalent protection than the MbΔmce2-phoP strain alone. Thus, the use of combined vaccination strategies requires a careful analysis of the potential interactions of each of their components with the host's immune system.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Contexto em Saúde:
1_ASSA2030
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2_ODS3
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3_ND
Problema de saúde:
1_doencas_transmissiveis
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2_enfermedades_transmissibles
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3_neglected_diseases
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3_tuberculosis
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3_zoonosis
Assunto principal:
Tuberculose
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Tuberculose Bovina
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Vacinas contra a Tuberculose
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Mycobacterium bovis
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Mycobacterium tuberculosis
Limite:
Animals
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Humans
Idioma:
En
Revista:
Int J Mycobacteriol
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Argentina