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A comprehensive approach using multiple biomarkers to detect damage induced by pesticides in broad-snouted caiman (Caiman latirostris) under ex-situ conditions.
López González, E C; Odetti, L M; Latorre, M A; Ávila, O B; Contini, L E; Siroski, P A; Poletta, G L.
Afiliação
  • López González EC; Proyecto Yacaré, Laboratorio de Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/Gob. Sta. Fe/MAyCC), Av. Aristóbulo del Valle 8700 (3000) Santa Fe, Argentina.
  • Odetti LM; Laboratorio de Ecología Molecular Aplicada (LEMA-Instituto de Ciencias Veterinarias del Litoral - Consejo Nacional de Investigaciones Científicas y Técnicas: ICiVet Litoral - CONICET/UNL), Av. Aristóbulo del Valle 8700 (3000) Santa Fe, Argentina.
  • Latorre MA; Cátedra de Toxicología Farmacología y Bioquímica Legal, FBCB-UNL, CONICET, Ciudad Universitaria, Paraje El Pozo S/N (3000) Santa Fe, Argentina.
  • Ávila OB; Proyecto Yacaré, Laboratorio de Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/Gob. Sta. Fe/MAyCC), Av. Aristóbulo del Valle 8700 (3000) Santa Fe, Argentina.
  • Contini LE; Departamento de Matemática, FBCB-UNL, Ciudad Universitaria, Paraje El Pozo S/N (3000) Santa Fe, Argentina.
  • Siroski PA; Departamento de Matemática, FBCB-UNL, Ciudad Universitaria, Paraje El Pozo S/N (3000) Santa Fe, Argentina.
  • Poletta GL; Proyecto Yacaré, Laboratorio de Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/Gob. Sta. Fe/MAyCC), Av. Aristóbulo del Valle 8700 (3000) Santa Fe, Argentina.
Heliyon ; 8(1): e08667, 2022 Jan.
Article em En | MEDLINE | ID: mdl-35028453
ABSTRACT
Caiman latirostris is one of the two species of the order Crocodylia that inhabit Argentina and is considered a species of vital ecological and economic importance in the north-east of Argentina. In this region, pesticides are the most common contaminants in natural environments and wild caiman populations are subject to this contamination constantly. The aim of this study was to evaluate the effects the main pesticides used in the region glyphosate (GLY), cypermethrin (CYP) and chlorpyrifos (CPF) -based formulations, as well as the mixture of them, on C. latirostris juveniles under semi-controlled condition of exposure (ex-situ) during 75 days. One hundred yearling caimans (10-month-old) were equally distributed into five experimental groups (20 animals per group) a negative control (NC -tap water), GLY 2% (Roundup® Full II formulation -RU), CYP 0.12% (Atanor® formulation), CPF 0.8% (Lorsban® formulation), and a mixture of the three pesticides (Mx3 GLY 2% + CYP 0.12% + CPF 0.8%). We applied early warning biomarkers to detect damage induced by these chemicals in peripheral blood activity of the antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD), analysis of lipid peroxidation (LPO) by the thiobarbituric acid reactive substances (TBARS), DNA damage and specific base oxidation through the standard and modified comet assay (CA), chromosome damage by micronucleus (MN) test and other nuclear abnormalities (NAs), hematological and growth parameters. Results showed a statistically significant increase in MN and NAs frequency, DNA damage, with an important contribution of base oxidation for all exposed groups compared to the NC. Total white blood cells count (TWBCC), and growth parameters showed effects mainly at the Mx3. The principal component analysis (PCA) demonstrated more sensitivity for biomarkers associated to genetic damage, including base oxidation to DNA than LPO, antioxidant enzyme modulation, immunotoxicity or growth parameters, to detect pesticides effects, applied under conditions similar to that found in natural environments.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Heliyon Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Argentina

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Heliyon Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Argentina
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