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MicroRNA detection using light-up aptamer amplification based on nuclease protection transcription.
Hong, Yoonseo; Kim, Do-Eun; Park, Yu-Jin; Kim, Dong-Myung; Byun, Ju-Young; Shin, Yong-Beom.
Afiliação
  • Hong Y; Bionanotechnology Research Center, Korea research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Korea. bjy8349@kribb.re.kr.
  • Kim DE; Department of Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 305-764, Korea.
  • Park YJ; Bionanotechnology Research Center, Korea research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Korea. bjy8349@kribb.re.kr.
  • Kim DM; Department of Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 305-764, Korea.
  • Byun JY; Department of Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 305-764, Korea.
  • Shin YB; Department of Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 305-764, Korea.
Chem Commun (Camb) ; 58(14): 2359-2362, 2022 Feb 15.
Article em En | MEDLINE | ID: mdl-35080531
ABSTRACT
The quantification of microRNAs (miRNAs) is important because the miRNA expression level is closely associated with the occurrence and development of diseases. Here, we report a simple nuclease protection transcription assay which combines nuclease protection assays and transcription-assisted light-up aptamer amplification for detecting miRNAs with great sensitivity.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Técnicas de Amplificação de Ácido Nucleico / MicroRNAs / Aptâmeros de Nucleotídeos Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Chem Commun (Camb) Assunto da revista: QUIMICA Ano de publicação: 2022 Tipo de documento: Article
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Técnicas de Amplificação de Ácido Nucleico / MicroRNAs / Aptâmeros de Nucleotídeos Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Chem Commun (Camb) Assunto da revista: QUIMICA Ano de publicação: 2022 Tipo de documento: Article