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Emergence of a highly colistin-resistant Aeromonas jandaei clinical isolate harbouring four genes encoding phosphoethanolamine transferases in Nepal.
Komeda, Tomoki; Shrestha, Shovita; Sherchan, Jatan B; Tohya, Mari; Hishinuma, Tomomi; Sherchand, Jeevan B; Tada, Tatsuya; Kirikae, Teruo.
Afiliação
  • Komeda T; Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan.
  • Shrestha S; Department of Microbiology, Institute of Medicine, Tribhuvan University, Kathmandu, Nepal.
  • Sherchan JB; Department of Clinical Microbiology, Kathmandu University School of Medical Sciences, Dhulikhel, Nepal.
  • Tohya M; Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan.
  • Hishinuma T; Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan.
  • Sherchand JB; Department of Microbiology, Institute of Medicine, Tribhuvan University, Kathmandu, Nepal.
  • Tada T; Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan. Electronic address: t-tada@juntendo.ac.jp.
  • Kirikae T; Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan.
Int J Antimicrob Agents ; 59(4): 106544, 2022 Apr.
Article em En | MEDLINE | ID: mdl-35134503
ABSTRACT

OBJECTIVES:

This study aimed to describe a clinical isolate of Aeromonas jandaei (A. jandaei) in Nepal that harboured four types of genes encoding phosphoethanolamine transferases.

METHODS:

An isolate of colistin-resistant A. jandaei was obtained from a blood sample of an inpatient in a hospital in Nepal, and its complete genome sequence was determined. Escherichia coli (E. coli) and Aeromonas hydrophila (A. hydrophila) transformants expressing genes encoding novel phosphoethanolamine transferase variants were constructed and colistin-susceptibility profiles were determined.

RESULTS:

The isolate harboured four genes encoding phosphoethanolamine transferases on the chromosome, which were designated eptAv3.2, eptAv3.3, eptAv3.4 and eptAv7.2. The amino acid sequences of EptAv3.2, 3.3 and 3.4 were > 80% identical to MCR-3.1, and that of EptAv7.2 was > 79% identical to MCR-7.1. E. coli expressing eptAv3.2, 3.3 and 3.4 showed reduced susceptibility to colistin, whereas E. coli expressing eptAv7.2 did not. In contrast, A. hydrophila expressing eptAv7.2 showed reduced susceptibility to colistin, whereas A. hydrophila expressing eptAv3.2, 3.3 and 3.4 did not; eptAv3.3 and 3.4 formed a tandem structure. The genomic environments surrounding eptAv3.2, 3.3 and 3.4 were similar to Aeromonas veronii obtained from the effluent of a treatment plant in Japan in 2018. The genomic environment surrounding eptAv7.2 was similar to that of A. jandaei obtained from a chicken in the USA in 2019.

CONCLUSIONS:

The highly colistin-resistant A. jandaei clinical isolate harboured four chromosomal genes encoding phosphoethanolamine transferases, suggesting that Aeromonas spp. harbouring eptAv genes with strong similarities to mcr-3 and mcr-7 are emerging in medical settings as well as environments.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Contexto em Saúde: 3_ND Problema de saúde: 3_neglected_diseases / 3_zoonosis Assunto principal: Aeromonas / Proteínas de Escherichia coli País/Região como assunto: Asia Idioma: En Revista: Int J Antimicrob Agents Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Japão

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Contexto em Saúde: 3_ND Problema de saúde: 3_neglected_diseases / 3_zoonosis Assunto principal: Aeromonas / Proteínas de Escherichia coli País/Região como assunto: Asia Idioma: En Revista: Int J Antimicrob Agents Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Japão
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