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Nanocrown electrodes for parallel and robust intracellular recording of cardiomyocytes.
Jahed, Zeinab; Yang, Yang; Tsai, Ching-Ting; Foster, Ethan P; McGuire, Allister F; Yang, Huaxiao; Liu, Aofei; Forro, Csaba; Yan, Zen; Jiang, Xin; Zhao, Ming-Tao; Zhang, Wei; Li, Xiao; Li, Thomas; Pawlosky, Annalisa; Wu, Joseph C; Cui, Bianxiao.
Afiliação
  • Jahed Z; Department of Chemistry and Stanford Wu-Tsai Neuroscience Institute, Stanford University, Stanford, CA, 94305, USA.
  • Yang Y; Department of Nanoengineering, Jacobs school of Engineering, University of California, San Diego, CA, 92039, USA.
  • Tsai CT; Department of Chemistry and Stanford Wu-Tsai Neuroscience Institute, Stanford University, Stanford, CA, 94305, USA.
  • Foster EP; Department of Chemistry and Stanford Wu-Tsai Neuroscience Institute, Stanford University, Stanford, CA, 94305, USA.
  • McGuire AF; Department of Chemistry and Stanford Wu-Tsai Neuroscience Institute, Stanford University, Stanford, CA, 94305, USA.
  • Yang H; Department of Chemistry and Stanford Wu-Tsai Neuroscience Institute, Stanford University, Stanford, CA, 94305, USA.
  • Liu A; Stanford Cardiovascular Institute, Stanford University, Stanford, CA, USA.
  • Forro C; Department of Biomedical Engineering, University of North Texas, Denton, TX, USA.
  • Yan Z; Department of Chemistry and Stanford Wu-Tsai Neuroscience Institute, Stanford University, Stanford, CA, 94305, USA.
  • Jiang X; Department of Chemistry and Stanford Wu-Tsai Neuroscience Institute, Stanford University, Stanford, CA, 94305, USA.
  • Zhao MT; Cyion Technologies, LLC, Pleasanton, CA, 94566, USA.
  • Zhang W; Cyion Technologies, LLC, Pleasanton, CA, 94566, USA.
  • Li X; Stanford Cardiovascular Institute, Stanford University, Stanford, CA, USA.
  • Li T; Center for Cardiovascular Research, Nationwide Children's Hospital, Columbus, OH, USA.
  • Pawlosky A; Department of Pediatrics, The Ohio State University College of Medicine, Columbus, OH, USA.
  • Wu JC; Department of Chemistry and Stanford Wu-Tsai Neuroscience Institute, Stanford University, Stanford, CA, 94305, USA.
  • Cui B; Department of Chemistry and Stanford Wu-Tsai Neuroscience Institute, Stanford University, Stanford, CA, 94305, USA.
Nat Commun ; 13(1): 2253, 2022 04 26.
Article em En | MEDLINE | ID: mdl-35474069
ABSTRACT
Drug-induced cardiotoxicity arises primarily when a compound alters the electrophysiological properties of cardiomyocytes. Features of intracellular action potentials (iAPs) are powerful biomarkers that predict proarrhythmic risks. In the last decade, a number of vertical nanoelectrodes have been demonstrated to achieve parallel and minimally-invasive iAP recordings. However, the large variability in success rate and signal strength have hindered nanoelectrodes from being broadly adopted for proarrhythmia drug assessment. In this work, we develop vertically-aligned nanocrown electrodes that are mechanically robust and achieve > 99% success rates in obtaining intracellular access through electroporation. We validate the accuracy of nanocrown electrode recordings by simultaneous patch clamp recording from the same cell. Finally, we demonstrate that nanocrown electrodes enable prolonged iAP recording for continual monitoring of the same cells upon the sequential addition of four incremental drug doses. Our technology development provides an advancement towards establishing an iAP screening assay for preclinical evaluation of drug-induced arrhythmogenicity.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Miócitos Cardíacos / Fenômenos Eletrofisiológicos Tipo de estudo: Prognostic_studies Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Miócitos Cardíacos / Fenômenos Eletrofisiológicos Tipo de estudo: Prognostic_studies Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos