Purification and cryo-EM structure determination of VCP/p97 dodecamers from mammalian and bacterial cells.

Yu, Guimei; Bai, Yunpeng; Zhang, Zhong-Yin

Yu, Guimei; Bai, Yunpeng; Zhang, Zhong-Yin.

Afiliação

Yu G; Departments of Medicinal Chemistry and Molecular Pharmacology and of Chemistry, Center for Cancer Research, and Institute for Drug Discovery, Purdue University, 720 Clinic Drive, West Lafayette, IN 47907, USA.
Bai Y; Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China.
Zhang ZY; Departments of Medicinal Chemistry and Molecular Pharmacology and of Chemistry, Center for Cancer Research, and Institute for Drug Discovery, Purdue University, 720 Clinic Drive, West Lafayette, IN 47907, USA.

STAR Protoc ; 3(2): 101339, 2022 06 17.

Article em En | MEDLINE | ID: mdl-35496809

Assuntos

Adenosina Trifosfatases; Proteínas de Ciclo Celular; Adenosina Trifosfatases/química; Animais; Proteínas de Ciclo Celular/química; Microscopia Crioeletrônica; Mamíferos/metabolismo; Proteína com Valosina/genética

Palavras-chave

Cryo-EM; Molecular Biology; Protein Biochemistry; Protein expression and purification; Structural Biology

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Adenosina Trifosfatases / Proteínas de Ciclo Celular Limite: Animals Idioma: En Revista: STAR Protoc Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos

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