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Vitamin D-induced LL-37 modulates innate immune responses of human primary macrophages during DENV-2 infection.
Castillo, Jorge Andrés; Giraldo, Diana Marcela; Smit, Jolanda M; Rodenhuis-Zybert, Izabela A; Urcuqui-Inchima, Silvio.
Afiliação
  • Castillo JA; Grupo Inmunovirología. Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Antioquia UdeA, Calle 70 No 52-21, Medellín, 050010, Colombia.
  • Giraldo DM; Department of Medical Microbiology and Infection Prevention, University of Groningen and University Medical Center Groningen, Groningen , The Netherlands.
  • Smit JM; Grupo Inmunovirología. Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Antioquia UdeA, Calle 70 No 52-21, Medellín, 050010, Colombia.
  • Rodenhuis-Zybert IA; Department of Medical Microbiology and Infection Prevention, University of Groningen and University Medical Center Groningen, Groningen , The Netherlands.
  • Urcuqui-Inchima S; Department of Medical Microbiology and Infection Prevention, University of Groningen and University Medical Center Groningen, Groningen , The Netherlands.
Pathog Dis ; 80(1)2022 05 23.
Article em En | MEDLINE | ID: mdl-35512569
ABSTRACT
Epidemics of dengue, an acute and potentially severe disease caused by mosquito-borne dengue virus (DENV), pose a major challenge to clinicians and health care services across the sub(tropics). Severe disease onset is associated with a dysregulated inflammatory response to the virus, and there are currently no drugs to alleviate disease symptoms. LL-37 is a potent antimicrobial peptide with a wide range of immunoregulatory properties. In this study, we assessed the effect of LL-37 on DENV-2-induced responses in human monocyte-derived macrophages (MDMs). We show that simultaneous exposure of exogenous LL-37 and DENV-2 resulted in reduced replication of the virus in MDMs, while the addition of LL-37 postexposure to DENV-2 did not. Interestingly, the latter condition reduced the production of IL-6 and increased the expression of genes involved in virus sensing and antiviral response. Finally, we demonstrate that low endogenous levels and limited production of LL-37 in MDMs in response to DENV-2 infection can be increased by differentiating MDMs in the presence of Vitamin D (VitD3). Taken together, this study demonstrates that in addition to its antimicrobial properties, LL-37 has immunomodulatory properties in the curse of DENV infection and its production can be increased by VitD3.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Contexto em Saúde: 2_ODS3 / 3_ND Problema de saúde: 2_enfermedades_transmissibles / 3_dengue / 3_neglected_diseases Assunto principal: Dengue / Vírus da Dengue Limite: Animals / Humans Idioma: En Revista: Pathog Dis Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Colômbia

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Contexto em Saúde: 2_ODS3 / 3_ND Problema de saúde: 2_enfermedades_transmissibles / 3_dengue / 3_neglected_diseases Assunto principal: Dengue / Vírus da Dengue Limite: Animals / Humans Idioma: En Revista: Pathog Dis Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Colômbia
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