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Filamentous bacteriophage delays healing of Pseudomonas-infected wounds.
Bach, Michelle S; de Vries, Christiaan R; Khosravi, Arya; Sweere, Johanna M; Popescu, Medeea C; Chen, Qingquan; Demirdjian, Sally; Hargil, Aviv; Van Belleghem, Jonas D; Kaber, Gernot; Hajfathalian, Maryam; Burgener, Elizabeth B; Liu, Dan; Tran, Quynh-Lam; Dharmaraj, Tejas; Birukova, Maria; Sunkari, Vivekananda; Balaji, Swathi; Ghosh, Nandini; Mathew-Steiner, Shomita S; El Masry, Mohamed S; Keswani, Sundeep G; Banaei, Niaz; Nedelec, Laurence; Sen, Chandan K; Chandra, Venita; Secor, Patrick R; Suh, Gina A; Bollyky, Paul L.
Afiliação
  • Bach MS; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA; Stanford Immunology, Stanford University, Stanford, CA 94305, USA.
  • de Vries CR; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA.
  • Khosravi A; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA.
  • Sweere JM; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA; Stanford Immunology, Stanford University, Stanford, CA 94305, USA.
  • Popescu MC; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA; Stanford Immunology, Stanford University, Stanford, CA 94305, USA.
  • Chen Q; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA.
  • Demirdjian S; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA.
  • Hargil A; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA.
  • Van Belleghem JD; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA.
  • Kaber G; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA.
  • Hajfathalian M; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA; Department of Radiology, University of Pennsylvania, Philadelphia, PA, USA.
  • Burgener EB; Center for Excellence in Pulmonary Biology, Department of Pediatrics, Stanford University, Stanford, CA 94305, USA.
  • Liu D; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA.
  • Tran QL; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA.
  • Dharmaraj T; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA; Stanford Immunology, Stanford University, Stanford, CA 94305, USA.
  • Birukova M; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA; Stanford Immunology, Stanford University, Stanford, CA 94305, USA.
  • Sunkari V; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA; Stanford Immunology, Stanford University, Stanford, CA 94305, USA.
  • Balaji S; Division of Pediatric Surgery, Department of Surgery, Baylor College of Medicine, Houston, TX 77030, USA.
  • Ghosh N; Department of Surgery, Indiana University, Indianapolis, IN 46202, USA.
  • Mathew-Steiner SS; Department of Surgery, Indiana University, Indianapolis, IN 46202, USA.
  • El Masry MS; Department of Surgery, Indiana University, Indianapolis, IN 46202, USA.
  • Keswani SG; Division of Pediatric Surgery, Department of Surgery, Baylor College of Medicine, Houston, TX 77030, USA.
  • Banaei N; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA; Division of Pathology, Department of Medicine, Stanford University, Stanford, CA 94305, USA.
  • Nedelec L; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA.
  • Sen CK; Department of Surgery, Indiana University, Indianapolis, IN 46202, USA.
  • Chandra V; Department of Surgery, Division of Vascular Surgery, Stanford University, Stanford, CA 94305, USA.
  • Secor PR; Division of Biological Sciences, University of Montana, Missoula, MT 59812, USA.
  • Suh GA; Division of Infectious Diseases, Mayo Clinic, Rochester, MN 55902, USA.
  • Bollyky PL; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA. Electronic address: pbollyky@stanford.edu.
Cell Rep Med ; 3(6): 100656, 2022 06 21.
Article em En | MEDLINE | ID: mdl-35732145
ABSTRACT
Chronic wounds infected by Pseudomonas aeruginosa (Pa) are characterized by disease progression and increased mortality. We reveal Pf, a bacteriophage produced by Pa that delays healing of chronically infected wounds in human subjects and animal models of disease. Interestingly, impairment of wound closure by Pf is independent of its effects on Pa pathogenesis. Rather, Pf impedes keratinocyte migration, which is essential for wound healing, through direct inhibition of CXCL1 signaling. In support of these findings, a prospective cohort study of 36 human patients with chronic Pa wound infections reveals that wounds infected with Pf-positive strains of Pa are more likely to progress in size compared with wounds infected with Pf-negative strains. Together, these data implicate Pf phage in the delayed wound healing associated with Pa infection through direct manipulation of mammalian cells. These findings suggest Pf may have potential as a biomarker and therapeutic target in chronic wounds.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por Pseudomonas / Infecção dos Ferimentos / Inovirus Tipo de estudo: Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Animals / Humans Idioma: En Revista: Cell Rep Med Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por Pseudomonas / Infecção dos Ferimentos / Inovirus Tipo de estudo: Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Animals / Humans Idioma: En Revista: Cell Rep Med Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos