The Level of LINE-1 mRNA Is Increased in Extracellular Circulating Plasma RNA in Patients with Colorectal Cancer.

Filipenko, M L; Boyarskikh, U A; Leskov, L S; Subbotina, K V; Khrapov, E A; Sokolov, A V; Stilidi, I S; Kushlinskii, N E

Filipenko, M L; Boyarskikh, U A; Leskov, L S; Subbotina, K V; Khrapov, E A; Sokolov, A V; Stilidi, I S; Kushlinskii, N E.

Afiliação

Filipenko ML; Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia. mlfilipenko@gmail.com.
Boyarskikh UA; Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
Leskov LS; M. F. Vladimirsky Moscow Regional Research Clinical Institute, Moscow, Russia.
Subbotina KV; Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
Khrapov EA; Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
Sokolov AV; City Clinical Hospital No. 1, Novosibirsk, Russia.
Stilidi IS; N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Moscow, Russia.
Kushlinskii NE; N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Moscow, Russia.

Bull Exp Biol Med ; 173(2): 261-264, 2022 Jun.

Article em En | MEDLINE | ID: mdl-35737160

ABSTRACT

ABSTRACT

We performed a comparative quantitative analysis of LINE-1 mRNA levels in extracellular total plasma RNA in patients with colon cancer and practically healthy donors. Quantitative multiplex PCR with reverse transcription was used to assess the level of LINE-1 and 18S rRNA mRNA in extracellular total plasma RNA. The median of LINE-1 mRNA values in colon cancer patients (4.95) was significantly higher than in healthy donors (2.3) (p=0.037). It was shown for the first time that the level of LINE-1 mRNA in total RNA from blood plasma can be determined in the format of a liquid biopsy and serve as a new potential non-invasive marker of colon cancer.

Assuntos

Ácidos Nucleicos Livres; Neoplasias do Colo; Neoplasias Colorretais; Proteínas de Ligação a RNA; Biomarcadores Tumorais/sangue; Biomarcadores Tumorais/genética; Ácidos Nucleicos Livres/sangue; Ácidos Nucleicos Livres/genética; Neoplasias do Colo/sangue; Neoplasias do Colo/genética; Neoplasias Colorretais/sangue; Neoplasias Colorretais/genética; Neoplasias Colorretais/patologia; Humanos; RNA Mensageiro/sangue; RNA Mensageiro/genética; Proteínas de Ligação a RNA/genética; Reação em Cadeia da Polimerase Via Transcriptase Reversa

Palavras-chave

LINE-1; colorectal cancer; freely circulating RNA; long dispersed nucleotide repeats (LINE); transcription

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Colorretais / Proteínas de Ligação a RNA / Neoplasias do Colo / Ácidos Nucleicos Livres Limite: Humans Idioma: En Revista: Bull Exp Biol Med Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Federação Russa

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