Detection of antibodies to Anaplasma phagocytophilum and Babesia microti using linear peptides.
Ticks Tick Borne Dis
; 13(5): 101999, 2022 09.
Article
em En
| MEDLINE
| ID: mdl-35816827
ABSTRACT
Anaplasma phagocytophilum and Babesia microti are emerging tick-borne pathogens in the United States. Although active infection is typically diagnosed by direct diagnostic tests, such as blood smear or polymerase chain reaction assay, serologic assays can be helpful to identify past infections, and the use of acute plus convalescent testing can potentially identify recent infections. We employed a peptide array to select sets of linear peptides for serologic diagnosis of infections with A. phagocytophilum and B. microti. Three optimal peptides were selected for each agent based on their performance with clinical specimens. All three A. phagocytophilum peptides were located within the conserved fragments of the MSP2 antigen. Two B. microti peptides were located in the N terminus of the SA-1 antigen; the third was in the BMN 1-17 antigen. We found that these peptides can be a useful tool for detection of antibody reactivity to both of these pathogens.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Babesiose
/
Borrelia burgdorferi
/
Babesia microti
/
Anaplasma phagocytophilum
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Ticks Tick Borne Dis
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Estados Unidos