Structure elucidation and gene cluster annotation of the O-antigen of Halomonas titanicae TAT1 containing three residues of 2,3-diacetamido-2,3-dideoxy-D-glucuronic acid.
Carbohydr Res
; 521: 108650, 2022 Nov.
Article
em En
| MEDLINE
| ID: mdl-35998422
ABSTRACT
A halotolerant hydrocarbon-oxidizing bacterium Halomonas titanicae strain TAT1 was isolated from a petroleum reservoir. The O-polysaccharide (O-antigen) was isolated from the lipopolysaccharide of H. titanicae TAT1 and studied by component analyses and 1D and 2D NMR spectroscopy. The following structure of the repeating linear pentasaccharide O-unit, containing only aminosugars, was established â4)-ß-d-GlcpNAc3NAcA-(1 â 4)-ß-d-GlcpNAc3NAcA-(1 â 6)-α-d-GlcpNAc-(1 â 4)-ß-d-GlcpNAc3NAcA-(1 â 6)-α-d-GlcpNAc-(â, where d-GlcNAc3NAcA indicates 2,3-diacetamido-2,3-dideoxy-d-glucuronic acid. The O-antigen gene cluster was identified in the genome of H. titanicae TAT1 and compared with available database sequences. The genes revealed in the O-antigen gene cluster and the assigned functions of putative proteins were consistent with the established polysaccharide structure.
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MEDLINE
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Petróleo
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Antígenos O
Idioma:
En
Revista:
Carbohydr Res
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Federação Russa