PalmitoylRGD promotes the expression of dermalepidermal junction components in HaCaT cells.
Mol Med Rep
; 26(4)2022 Oct.
Article
em En
| MEDLINE
| ID: mdl-36043531
ABSTRACT
With age, the dermalepidermal junction (DEJ) becomes thinner and production of its protein components decreases; this may be associated with increased fragility and wrinkling of skin. Topical treatment with palmitoylArgGlyAsp (PALRGD) improves facial wrinkles, skin elasticity and dermal density in humans. In the present study, the effect of PALRGD on expression of DEJ components, such as laminin and collagen, was assessed. Human HaCaT keratinocytes were treated with PALRGD. The protein expression levels of laminin332, collagen IV and collagen XVII were examined by western blotting. Reverse transcription-quantitative PCR was used to analyze laminin subunit (LAM)A3, LAMB3, LAMC2, collagen type IV α 1 chain (COL4A1) and COL17A1 mRNA expression levels. Western blot analysis showed that the expression levels of proteins comprising the DEJ, including laminin α3, ß3 and γ2 and collagen IV and XVII demonstrated a significant dosedependent increase following PALRGD treatment. Furthermore, PALRGD treatment significantly enhanced LAMA3, LAMB3, LAMC2, COL4A1 and COL17A1 mRNA expression levels. PALRGD may enhance the DEJ by inducing the expression of laminin332, collagen IV and collagen XVII.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Laminina
/
Células HaCaT
Limite:
Humans
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2022
Tipo de documento:
Article