Isolation and identification of Tussilago farfara leaf spot caused by Alternaria alternata in China.
Microb Pathog
; 172: 105750, 2022 Nov.
Article
em En
| MEDLINE
| ID: mdl-36075342
ABSTRACT
Tussilago farfara is of vital medical value. A new leaf spot disease was observed on T. farfara leaves, in Dingxi, Gansu Province, China, in October 2019. In order to research the pathogen, the diseased samples were collected for isolation and identification. The isolate KD3 was verified by pathogenicity test, as the pathogen causing the T. farfara leaf spot disease. Its morphological characteristics were consistent with Alternaria alternata, the colony color gray-green with concentric rings, conidia fusiform and pear-shaped, brown, with 1-7 septa and 0-3 longitudinal septa, conidia size (19. 62-44.49) µm × (6.97-10.53) µm, beak length (1.35-10.03) µm × (1.01-3.63) µm, and the spore phenotype was a dwarf tree-like chain of short conidia. Multilocus sequences analysis manifested that the internal transcribed spacer (ITS), Alternaria major allergen (Alta1), and Calcium barine (CAL) sequences of strain KD3 were most closely to A. alternata (A23), with the homology of 99.47%, 99.56% and 98.28%, respectively. Based on morphological and molecular characteristics, strain KD3 was identified as A. alternata. OA was the optimal medium for its growth and PCA medium was the optimal for sporulation. This is the first report of A. alternata causing T. farfara leaf spots in China.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tussilago
/
Alternaria
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
País/Região como assunto:
Asia
Idioma:
En
Revista:
Microb Pathog
Assunto da revista:
DOENCAS TRANSMISSIVEIS
/
MICROBIOLOGIA
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
China